Association of aster yellows subgroup 16SrI-C phytoplasmas with a disease of Ribes rubrum

0367360 - BC 2012 RIV IT eng J - Journal Article
Přibylová, Jaroslava - Petrzik, Karel - Špak, Josef
Association of aster yellows subgroup 16SrI-C phytoplasmas with a disease of Ribes rubrum.
Bulletin of Insectology. Roč. 64, Suppl. (2011), s. 65-66. ISSN 1721-8861. E-ISSN 2283-0332
R&D Projects: GA MZe QH91224
Institutional research plan: CEZ:AV0Z50510513
Keywords : PCR * RFLP analysis * red currant
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.592, year: 2011

A Ribes rubrum plant showing malformation and twisting of branches was found in a garden in South Bohemia. Ultrathin sections of tissues from symptomatic shoots revealed the presence of phytoplasma-like bodies. Different primer sets were used for amplification of the 16S-23S ribosomal gene segment. RFLP analysis and sequencing for phytoplasma identification classified the detected phytoplasma in the aster yellows group, subgroup 16SrI-C. Successful transmission of detected phytoplasma by dodder to periwinkle was confirmed by detection of numerous phytoplasma bodies in ultrathin sections of C. roseus and by PCR from dodder and periwinkle. RFLP analyses of PCR products as well as nucleotide sequences of the currant plant and symptomatic periwinkles were identical. Sequenced data obtained from both currant and indicator plant, were aligned and sequences of 1,613 bp were found to be identical. Transmissions of phytoplasma by grafting to healthy currant rootstocks were unsuccessful.
Permanent Link: http://hdl.handle.net/11104/0202069