Discriminating 16Sr groups of phytoplasma by an oligonucleotide microarray targeting 16S-23S ribosomal spacer

0367359 - BC 2012 RIV IT eng J - Journal Article
Lenz, Ondřej - Marková, J. - Sarkisova, Tatiana
Discriminating 16Sr groups of phytoplasma by an oligonucleotide microarray targeting 16S-23S ribosomal spacer.
Bulletin of Insectology. Roč. 64, Suppl. (2011), s. 31-32. ISSN 1721-8861. E-ISSN 2283-0332
R&D Projects: GA ČR GP522/09/P545
Institutional research plan: CEZ:AV0Z50510513
Keywords : phytoplasmas * detection * 16Sr groups * ribosomal spacer * microarray
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.592, year: 2011

A microarray detecting phytoplasma 16Sr groups I, II, III, V, VI, X and XII was developed based on probes targeting 16S-23S ribosomal spacer. Despite it is considered as more suitable than 16S rDNA for the subtle species determination, the microarray did not demonstrate discriminatory potential higher than the only phytoplasma microarray published so far based on 16S rDNA. Nevertheless, methods used were cheaper and faster than in the latter bringing the microarray towards the real routine analysis.
Permanent Link: http://hdl.handle.net/11104/0202068