Nanoproteomics : Methods and Protocols

0367283 - ÚIACH 2012 RIV US eng M - Monography Chapter
Vitorino, L. - Křenková, Jana - Foret, František - Domingues, P. - Amado, F.
Protein identification using nano-HPLC-MS: ESI-MS and MALDI-MS interfaces.
Nanoproteomics : Methods and Protocols. New York: Humana Press, 2011 - (Toms, S.; Weil, R.), s. 31-46. Methods in Molecular Biology, 790. ISBN 978-1-61779-318-9
R&D Projects: GA ČR(CZ) GAP301/11/2055; GA ČR(CZ) GPP206/11/P004
Institutional research plan: CEZ:AV0Z40310501
Keywords : liquid chromatography * mass spectrometry * proteomics
Subject RIV: CB - Analytical Chemistry, Separation

We describe the protocols to characterize high molecular weight peptides and intact proteins involving on-line trypsin digestion and separation of digest using nano-HPLC with mass spectrometry detection.
Permanent Link: http://hdl.handle.net/11104/0202022