Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping

0367249 - BC 2012 RIV NL eng J - Journal Article
Patzak, J. - Matoušek, Jaroslav
Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping.
Biologia Plantarum. Roč. 55, č. 4 (2011), s. 761-765. ISSN 0006-3134. E-ISSN 1573-8264
R&D Projects: GA ČR GA521/08/0740; GA MZe QH81052
Institutional research plan: CEZ:AV0Z50510513
Keywords : cluster analysis * dendrogram * Humulus lupulus * simple sequence repeat
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.974, year: 2011

The use of expressed sequence tag-simple sequence repeat (EST-SSR) markers might reflect the better relationship among species or cultivars than markers previously used. The first set of 30 EST-SSR was developed in hop (Humulus lupulus L.). They represent 25 gene loci with total of 1268 EST sequences. They were used for characterization of 11 hop samples and cross-amplification in Humulus japonicus Sieb. et Zucc. The number of alleles per locus ranged from two to nine. The observed and expected heterozygosities ranged from 0.182 to 0.956 and from 0.233 to 0.775, respectively. We used EST-SSR markers for cluster analysis of hop genotypes. Dendrogram well matched with genealogical and geographical data for hop genotypes.
Permanent Link: http://hdl.handle.net/11104/0201993