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Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella (Lepidoptera: Pyralidae)

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    0367049 - BFÚ 2012 RIV CZ eng J - Journal Article
    Vašíček, Ondřej - Papežíková, Ivana - Hyršl, P.
    Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella (Lepidoptera: Pyralidae).
    European Journal of Entomology. Roč. 108, č. 3 (2011), s. 481-485. ISSN 1210-5759. E-ISSN 1802-8829
    Grant - others:GA ČR(CZ) GP206/09/P470
    Program: GP
    Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
    Keywords : Galleria mellonella * fluorescence * hydrogen peroxide
    Subject RIV: BO - Biophysics
    Impact factor: 1.061, year: 2011

    Production of ROS, as an important tool for oxidative killing of invading pathogens during phagocytosis. In this study Amplex red reagent, an ultrasensitive method for detection the production of H2O2, is used to provide evidence of H2O2 production by the haemocytes of Galleria mellonella larvae. The results show that the haemocytes of G. mellonella produced a low level of ROS.
    Permanent Link: http://hdl.handle.net/11104/0006658

     
     
Number of the records: 1  

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