Translation Reinitiation Relies on the Interaction between eIF3a/TIF32 and Progressively Folded cis-Acting mRNA Elements Preceding Short uORFs

0366863 - MBÚ 2012 RIV US eng J - Journal Article
Munzarová, Vanda - Pánek, Josef - Gunišová, Stanislava - Dányi, István - Szamecz, Bela - Valášek, Leoš
Translation Reinitiation Relies on the Interaction between eIF3a/TIF32 and Progressively Folded cis-Acting mRNA Elements Preceding Short uORFs.
PLoS Genetics. Roč. 7, č. 7 (2011), s. 1-16. ISSN 1553-7390
R&D Projects: GA ČR GA303/09/0475
Institutional research plan: CEZ:AV0Z50200510
Keywords : OPEN READING FRAMES * INITIATION-FACTORS * IN-VIVO
Subject RIV: EE - Microbiology, Virology
Impact factor: 8.694, year: 2011

Reinitiation is a gene-specific translational control mechanism characterized by the ability of some short upstream uORFs to tain post-termination 40S subunits on mRNA. Its efficiency depends on surrounding cis-acting sequences, uORF elongation rates, various initiation factors, and the intercistronic distance. To unravel effects of cis-acting sequences, we investigated previously unconsidered structural properties of one such a cis-enhancer in the mRNA leader of GCN4 using yeast genetics and biochemistry. This leader contains four uORFs but only uORF1, flanked by two transferrable 59 and 39 cisacting sequences, and allows efficient reinitiation. Recently we showed that the 59 cis-acting sequences stimulate reinitiation by interacting with the N-terminal domain (NTD) of the eIF3a/TIF32 subunit of the initiation factor eIF3 to stabilize post-termination 40S subunits on uORF1 to resume scanning downstream
Permanent Link: http://hdl.handle.net/11104/0201704