Molecular characterization of the iron binding protein ferritin in Eisenia andrei earthworms

0366799 - MBÚ 2012 RIV NL eng J - Journal Article
Procházková, Petra - Dvořák, Jiří - Šilerová, Marcela - Roubalová, Radka - Škanta, František - Halada, Petr - Bilej, Martin
Molecular characterization of the iron binding protein ferritin in Eisenia andrei earthworms.
Gene. Roč. 485, č. 2 (2011), s. 73-80. ISSN 0378-1119. E-ISSN 1879-0038
R&D Projects: GA ČR GA206/07/0378; GA ČR GD310/08/H077; GA AV ČR IAA600200704; GA MŠMT 2B06155; GA MŠMT LC07017
Institutional research plan: CEZ:AV0Z50200510
Keywords : Earthworms * Invertebrates * Ferritin
Subject RIV: EC - Immunology
Impact factor: 2.341, year: 2011

Ferritin is a storage protein that plays a key role in iron metabolism. In this study, we report on the sequence characterization of a ferritin-coding cDNA in Eisenia andrei earthworms isolated by RT-PCR using degenerated primers, and we suggest the presence of a putative IRE in the 5′-UTR of ferritin mRNA. The obtained ferritin sequence was compared with those of other animals showing sequence and structure homology in consensus sites, including the iron-responsive element (IRE) and ferroxidase centers. Despite the sequence homology in the E. andrei mRNA of ferritin with the sequences of other animals in consensus IRE sites, the presented cytosine in the IRE of E. andrei ferritin in the expected position does not form a conventional bulge
Permanent Link: http://hdl.handle.net/11104/0201662