Protonation of Lipids Impacts the Supramolecular and Biological Properties of Their Self-Assembly

0366666 - ÚFCH JH 2012 RIV US eng J - Journal Article
Breton, M. - Berret, J. - F. - Bourgaux, C. - Kral, Teresa - Hof, Martin - Pichon, Ch. - Bessodes, M. - Scherman, D. - Mignet, N.
Protonation of Lipids Impacts the Supramolecular and Biological Properties of Their Self-Assembly.
Langmuir. Roč. 27, č. 20 (2011), s. 12336-12345. ISSN 0743-7463
R&D Projects: GA AV ČR IAA400400621; GA MŠMT(CZ) LC06063
Institutional research plan: CEZ:AV0Z40400503
Keywords : fluorescence correlation spectroscopy * angle neutron-scattering * bilayer thickness
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 4.186, year: 2011

We assessed in this work how a chemical structure difference could influence a supramolecular organization and then its biological properties. In our case study, we considered two amphiphilic lipidic gene vectors. The chemical difference was situated on their hydrophilic part which was either a pure neutral thiourea head or a mixture of three thiourea function derivatives, thiourea, iminothiol, and charged iminothiol. This small difference was obtained thanks to the last chemical deprotection conditions of the polar head hydroxyl groups. Light, neutron, and X-ray scattering techniques have been used to investigate the spatial structure of the liposomes and lipoplexes formed by the lipids. The chemical structure difference impacts the supramolecular assemblies of the lipids and with DNA as shown by fluorescence correlation spectroscopy (FCS), X-ray, and neutron scattering.
Permanent Link: http://hdl.handle.net/11104/0006643