Enrichment techniques employed in phosphoproteomics

0366394 - ÚEB 2013 RIV AT eng J - Journal Article
Fíla, Jan - Honys, David
Enrichment techniques employed in phosphoproteomics.
Amino Acids. Roč. 43, č. 3 (2012), s. 1025-1047. ISSN 0939-4451. E-ISSN 1438-2199
R&D Projects: GA ČR(CZ) GAP501/11/1462; GA ČR GA522/09/0858; GA ČR GA525/09/0994; GA MŠMT OC08011
Institutional research plan: CEZ:AV0Z50380511
Keywords : Phosphoproteomics * Enrichment * IMAC
Subject RIV: ED - Physiology
Impact factor: 3.914, year: 2012

Rapid changes of protein phosphorylation play a crucial role in the regulation of many cellular processes. Being post-translationally modified, phosphoproteins are often present in quite low abundance and tend to co-exist with their unphosphorylated isoform within the cell. To make their identification more practicable, the use of enrichment protocols is often required. The enrichment strategies can be performed either at the level of phosphoproteins or at the level of phosphopeptides. Both approaches have their advantages and disadvantages. Most enriching strategies are based on chemical modifications, affinity chromatography to capture peptides and proteins containing negatively charged phosphate groups onto a positively charged matrix, or immunoprecipitation by phospho-specific antibodies. In this article, the most up-to-date enrichment techniques are discussed, taking into account their optimization, and highlighting their advantages and disadvantages.
Permanent Link: http://hdl.handle.net/11104/0201412