Characterization of a new 4BS.7HL wheat–barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat

0366032 - ÚEB 2012 RIV CA eng J - Journal Article
Cseh, A. - Kruppa, K. - Molnár, I. - Rakszegi, M. - Doležel, Jaroslav - Molnár-Láng, M.
Characterization of a new 4BS.7HL wheat–barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat.
Genome. Roč. 54, č. 10 (2011), s. 795-804. ISSN 0831-2796. E-ISSN 1480-3321
Institutional research plan: CEZ:AV0Z50380511
Keywords : wheat–barley chromosome translocation * β-d-glucan * centromere
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.653, year: 2011

A spontaneous interspecific Robertsonian translocation was revealed by genomic in situ hybridization (GISH) in the progenies of a monosomic 7H addition line originating from a new wheat ‘Asakaze komugi’ × barley ‘Manas’ hybrid. Fluorescence in situ hybridization (FISH) with repetitive DNA sequences (Afa family, pSc119.2, and pTa71) allowed identification of all wheat chromosomes, including wheat chromosome arm 4BS involved in the translocation. FISH using barley telomere- and centromere-specific repetitive DNA probes (HvT01 and (AGGGAG)n) confirmed that one of the arms of barley chromosome 7H was involved in the translocation. Simple sequence repeat (SSR) markers specific to the long (L) and short (S) arms of barley chromosome 7H identified the translocated chromosome segment as 7HL. The presence of the HvCslF6 gene, responsible for (1,3;1,4)-b-D-glucan production, was revealed in the centromeric region of 7HL.
Permanent Link: http://hdl.handle.net/11104/0201134