Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues

0365995 - BC 2012 RIV GB eng J - Journal Article
Long, Shaojun - Changmai, Piya - Tsaousis, A.D. - Skalický, Tomáš - Verner, Zdeněk - Wen, Yan-Zi - Roger, A. J. - Lukeš, Julius
Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues.
Molecular Microbiology. Roč. 81, č. 6 (2011), 1403-1418. ISSN 0950-382X. E-ISSN 1365-2958
R&D Projects: GA ČR GA204/09/1667; GA MŠMT LC07032; GA MŠMT 2B06129
Institutional research plan: CEZ:AV0Z60220518
Keywords : IRON-SULFUR CLUSTER * ESCHERICHIA-COLI * ASSEMBLY PROTEIN * SACCHAROMYCES-CEREVISIAE * AZOTOBACTER-VINELANDII * CYSTEINE DESULFURASE * CRYSTAL-STRUCTURE * BINDING ACTIVITY * GENE-CLUSTER
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 5.010, year: 2011

IscA/Isa proteins function as alternative scaffolds for the assembly of Fe-S clusters and/or provide iron for their assembly in prokaryotes and eukaryotes. We have studied their function in Trypanosoma brucei, where the requirement for both of them to sustain cell growth depends on the life cycle stage. Reactive oxygen species but not iron accumulate in the procyclic mitochondrion upon ablation of the TbIsa proteins, but their depletion does not influence the assembly of Fe-S clusters in cytosolic proteins. In the bloodstream form, which has a downregulated mitochondrion, the TbIsa proteins are non-essential. The Isa2 orthologue of the anaerobic protist Blastocystis partially rescued the growth and enzymatic activities of TbIsa1/2 knock-down. Rescues with human Isa orthologues partially recovered the activities of aconitase and fumarase. These results show that the Isa1 and Isa2 proteins of diverse eukaryotes have overlapping functions.
Permanent Link: http://hdl.handle.net/11104/0201106