Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes

0365945 - ÚEB 2012 RIV NL eng J - Journal Article
Zatloukalová, Pavlína - Hřibová, Eva - Kubaláková, Marie - Suchánková, Pavla - Šimková, Hana - Adoración, C. - Kahl, G. - Millán, T. - Doležel, Jaroslav
Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes.
Chromosome Research. Roč. 19, č. 6 (2011), s. 729-739. ISSN 0967-3849. E-ISSN 1573-6849
R&D Projects: GA MŠMT(CZ) LC06004
Institutional research plan: CEZ:AV0Z50380511
Keywords : BAC-FISH * Chromosome isolation * Flow cytometric sorting
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.087, year: 2011

Various genetic linkage maps have been established and markers linked to resistance genes been identified in cultivated chickpea. However, until now, only one linkage group (LG) has been assigned to a specific chromosome. In the present work, mitotic chromosomes were sorted using flow cytometry and used as template for PCR with primers designed for genomic regions flanking microsatellites. These primers amplify sequence-tagged microsatellite site markers. To establish a set of preferably diagnostic cytogenetic markers, the genomic distribution of various probes was verified using FISH. Moreover, a partial genomic bacterial artificial chromosome (BAC) library was constructed and putative single/low-copy BAC clones weremapped cytogenetically. As a result, two clones were identified localizing specifically to chromosomes E and H, for which no cytogenetic markers were yet available.
Permanent Link: http://hdl.handle.net/11104/0201070