A platform for efficient genotyping in Musa using microsatellite markers

0365578 - ÚEB 2012 RIV GB eng J - Journal Article
Christelová, Pavla - Valárik, Miroslav - Hřibová, Eva - Van den Houwe, I. - Channeliere, S. - Roux, N. - Doležel, Jaroslav
A platform for efficient genotyping in Musa using microsatellite markers.
AoB PLANTS. Roč. 2011, - (2011), s. 1-14. ISSN 2041-2851. E-ISSN 2041-2851
Institutional research plan: CEZ:AV0Z50380511
Keywords : Musa * microsatellite markers * Cassava
Subject RIV: EB - Genetics ; Molecular Biology
http://arl-repository.lib.cas.cz/uloziste_av/UEB-Q/cav_un_epca-0365578_01.pdf

With the aim of providing a simple and robust approach for molecular characterization of Musa species, we developed an optimized genotyping platform using 19 published simple sequence repeat markers. The genotyping system is based on 19 microsatellite loci, which are scored using fluorescently labelled primers and high-throughput capillary electrophoresis separation with high resolution. This genotyping platform was tested and optimized on a set of 70 diploid and 38 triploid banana accessions. The marker set used in this study provided enough polymorphism to discriminate between individual species, subspecies and subgroups of all accessions of Musa. Likewise, the capability of identifying duplicate samples was confirmed. Based on the results of a blind test, the genotyping system was confirmed to be suitable for characterization of unknown accessions. Here we report on the first complex and standardized platform for molecular characterization of Musa germplasm.
Permanent Link: http://hdl.handle.net/11104/0200786