Assessment of Ploidy and Genome Constitution of Some Musa balbisiana Cultivars using DArT Markers

0365265 - ÚEB 2012 RIV PH eng J - Journal Article
Sales, E. K. - Butardo, N. G. - Paniagua, H. G. - Jansen, H. - Doležel, Jaroslav
Assessment of Ploidy and Genome Constitution of Some Musa balbisiana Cultivars using DArT Markers.
Philippine Journal of Crop Science. Roč. 36, č. 1 (2011), s. 11-18. ISSN 0115-463X
Institutional research plan: CEZ:AV0Z50380511
Keywords : DArT * genome * Musa balbisiana
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.075, year: 2011
http://home.ueb.cas.cz/publikace/2011_Sales_PHILIPPINE_JOURNAL_OF_CROP_SCIENCE_11.pdf http://arl-repository.lib.cas.cz/uloziste_av/UEB-Q/cav_un_epca-0365265_01.pdf http://arl-repository.lib.cas.cz/uloziste_av/UEB-Q/cav_un_epca-0365265_02.pdf

The Philippines is one of the centers of origin and diversity for bananas. There is an endless range of names and synonyms for cultivars, thus the major constraint in banana and plantain research is on proper identification and classification. Musa balbisiana is grown extensively and considered endemic in the Philippines. However, there are opposing views as to the genomic constitution of cultivars. Philippine and other Asian Musa experts claimed that Philippine 'Saba' group is of the triploid B but American and European Musa workers denied the existence of such and consistently claimed that it is hybrids of A and B genomes. The objective of the study was to determine if Diversity Array Technology (DArT) analysis can efficiently verify the genome constitution of the Musa cultivars in question. Twenty-four Philippine Musa cultivars were subjected to flow cytometry analysis for ploidy confirmation and DArT analysis for molecular characterization.
Permanent Link: http://hdl.handle.net/11104/0200548