Mouse 3T3 fibroblasts under the influence of fibroblasts isolated from stroma of human basal cell carcinoma acquire properties of multipotent stem cells

0365189 - ÚMG 2012 RIV FR eng J - Journal Article
Szabo, Pavol - Kolář, Michal - Dvořánková, B. - Lacina, L. - Štork, J. - Vlček, Čestmír - Strnad, Hynek - Tvrdek, M. - Smetana, K.
Mouse 3T3 fibroblasts under the influence of fibroblasts isolated from stroma of human basal cell carcinoma acquire properties of multipotent stem cells.
Biology of the Cell. Roč. 103, č. 5 (2011), s. 233-248. ISSN 0248-4900. E-ISSN 1768-322X
R&D Projects: GA MŠMT 2B06106; GA MŠMT(CZ) 1M0520
Institutional research plan: CEZ:AV0Z50520514
Keywords : basal cell carcinoma (BCC) * stem cell * tumour stroma
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.600, year: 2011

Multipotent mesenchymal stem cells can participate in the formation of a microenvironment stimulating the aggressive behaviour of cancer cells. Cells exhibiting pluripotent embryonic stem cell markers (Nanog and Oct4) have been observed in many tumours. Here, we investigate the stem cell supporting role of cancer-associated fibroblasts (CAF). We test the influence of CAF isolated from basal cell carcinoma on mouse 3T3 fibroblasts, focusing on the expression of stem cell markers and plasticity in vitro. We demonstrate the biological activity of CAF by influencing the 3T3 fibroblasts to express markers such as Oct4, Nanog and Sox2 and to show differentiation potential similar to mesenchymal stem cells. The role of growth factors such as IGF2, FGF7, LEP, NGF and TGFβ, produced by CAF, is established to participate in their activity. Our observations indicate the pro-stem cell activity of CAF and underline the role of epithelial–mesenchymal interaction in tumour biology.
Permanent Link: http://hdl.handle.net/11104/0200489