Interaction of late apoptotic and necrotic cells with vitronectin

0365171 - ÚMG 2012 RIV US eng J - Journal Article
Štěpánek, Ondřej - Brdička, Tomáš - Angelisová, Pavla - Horváth, Ondřej - Špička, Jiří - Stockbauer, P. - Man, Petr - Hořejší, Václav
Interaction of late apoptotic and necrotic cells with vitronectin.
PLoS ONE. Roč. 6, č. 5 (2011), e19243. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA MŠMT 1M0506
Institutional research plan: CEZ:AV0Z50200510; CEZ:AV0Z50520514
Keywords : vitronectin * apoptosis * necrosis
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.092, year: 2011

Vitronectin (Vn) is an abundant plasma glycoprotein identified also as a part of extracellular matrix. Vn is substantially enriched at sites of injured, fibrosing, inflamed, and tumor tissues where it is believed to be involved in wound healing and tissue remodeling. Little is known about the mechanism of Vn localization into the damaged tissues. 2E12 antibody has been described to bind a subset of late apoptotic cells. The antigen recognized by 2E12 antibody was identified as Vn. Vn binds to the late apoptotic and necrotic cells in cell cultures in vitro as well as in murine thymus and spleen in vivo. Confocal microscopy revealed that Vn binds to an intracellular cytoplasmic structure after the membrane rupture. Vitronectin could serve as a marker of membrane disruption in necrosis and apoptosis for flow cytometry analysis. Vn binding to dead cells may represent one of the mechanisms of Vn incorporation into the injured tissues.
Permanent Link: http://hdl.handle.net/11104/0200476