PRR7 Is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis

0365069 - ÚMG 2012 RIV US eng J - Journal Article
Hrdinka, Matouš - Dráber, Peter - Štěpánek, Ondřej - Ormsby, Tereza - Otáhal, Pavel - Angelisová, Pavla - Brdička, Tomáš - Pačes, Jan - Hořejší, Václav - Drbal, Karel
PRR7 Is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis.
Journal of Biological Chemistry. Roč. 286, č. 22 (2011), s. 19617-19629. ISSN 0021-9258. E-ISSN 1083-351X
R&D Projects: GA MŠMT 1M0506
Grant - others:GAČR(CZ) MEM/09/E011
Institutional research plan: CEZ:AV0Z50520514
Keywords : PRR7 * transmembrane adaptor protein * apoptosis
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.773, year: 2011

A highly conserved ransmembrane adaptor protein (TRAP) PRR7 was found to be readily up-regulated in activated human peripheral blood lymphocytes. Transient overexpression of PRR7 in Jurkat T cell line led to gradual apoptotic death dependent on the WW domain binding motif surrounding Tyr-166 in the intracellular part of PRR7. Jurkat clones with inducible expression of PRR7 (J-iPRR7) were generated. In these cells acute induction of PRR7 expression had a dual effect. It resulted in up-regulation of the transcription factor c-Jun and the activation marker CD69 as well as enhanced production of IL-2 after phorbol 12-myristate 13-acetate (PMA) and ionomycin treatment. Expression of PRR7 inhibited general tyrosine phosphorylation and calcium influx after T cell receptor cross-linking by antibodies. PRR7 was constitutively tyrosine-phosphorylated and associated with Src. PRR7 is a potential regulator of signaling and apoptosis in activated T cells.
Permanent Link: http://hdl.handle.net/11104/0200405