Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies

0364996 - ÚMCH 2012 RIV GB eng J - Journal Article
Angelov, Borislav - Angelova, A. - Filippov, Sergey K. - Karlsson, G. - Terrill, N. - Lesieur, S. - Štěpánek, Petr
Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies.
Soft Matter. Roč. 7, č. 20 (2011), s. 9714-9720. ISSN 1744-683X. E-ISSN 1744-6848
R&D Projects: GA ČR GA202/09/2078; GA ČR GAP208/10/1600
Institutional research plan: CEZ:AV0Z40500505
Keywords : DNA-lipid-surfactant complexes * CryoTEM * small-angle X-ray scattering
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 4.390, year: 2011

Towards potential treatment of neurodegenerative disorders by nanomedicine gene therapy, a nonviral gene delivery system is created by complexation of novel PEGylated cationic lipid assemblies and a plasmid DNA encoding for the protein brain-derived neurotrophic factor (BDNF). The generated monoolein-based liquid crystalline nanocarriers for BDNF gene transfer are characterized by means of small-angle X-ray scattering (SAXS), cryogenic transmission electron microscopy (cryo-TEM) techniques, and turbidity measurements. Morphologically variable populations of sterically stabilized, PEGylated multicompartment nanoobjects, with confined supercoiled DNA, are established by cryo-TEM imaging. Synchrotron radiation SAXS measurements, performed with diluted nanoparticulate dispersions, evidence the inner multilamelar structure of pear-type vesicles and flexible onion-like objects functionalized by pBDNF. Obtained structural data are of fundamental interest for improvement of brain-targeted gene delivery.
Permanent Link: http://hdl.handle.net/11104/0200341