Alboserpin, a Factor Xa Inhibitor from the Mosquito Vector of Yellow Fever, Binds Heparin and Membrane Phospholipids and Exhibits Antithrombotic Activity

0364839 - BC 2012 RIV US eng J - Journal Article
Calvo, E. - Mizurini, D.M. - Sa-Nunes, A. - Ribeiro, J.M.C. - Andersen, J. F. - Mans, B.J. - Monteiro, R.Q. - Kotsyfakis, Michalis - Francischetti, I.M.B.
Alboserpin, a Factor Xa Inhibitor from the Mosquito Vector of Yellow Fever, Binds Heparin and Membrane Phospholipids and Exhibits Antithrombotic Activity.
Journal of Biological Chemistry. Roč. 286, č. 32 (2011), 27998-28010. ISSN 0021-9258. E-ISSN 1083-351X
Institutional research plan: CEZ:AV0Z60220518
Keywords : serpin * mosquito * Aedes albopictus * phospholipids * Factor Xa * heparin * binding affinity * coagulation * thrombus * bleeding
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 4.773, year: 2011

The function of a mosquito anticoagulant serpin named as Alboserpin is presented. The recombinant protein binds to phospholipids and it inhibits the activity of human coagulation factor Xa. Binding is highly specific and is not detectable for FX, catalytic site-blocked FXa, thrombin and 12 other enzymes. Alboserpin displays high-affinity binding to heparin, but no change in FXa inhibition was observed in the presence of the cofactor, implying that bridging mechanisms did not take place. Alboserpin displays a potent pharmacological activity since it prevents thrombus formation provoked by ferric chloride injury of the carotid artery and it increases bleeding in a dose-dependent manner.
Permanent Link: http://hdl.handle.net/11104/0200216