Neoparamoeba branchiphila infections in moribund sea urchins Diadema aff. antillarum in Tenerife, Canary Islands, Spain

0364749 - BC 2012 RIV DE eng J - Journal Article
Dyková, Iva - Lorenzo-Morales, J. - Kostka, Martin - Valladares, B. - Pecková, Hana
Neoparamoeba branchiphila infections in moribund sea urchins Diadema aff. antillarum in Tenerife, Canary Islands, Spain.
Diseases of Aquatic Organisms. Roč. 95, č. 3 (2011), 225-231. ISSN 0177-5103. E-ISSN 1616-1580
R&D Projects: GA ČR GA524/09/0137; GA MŠMT LC522
Institutional research plan: CEZ:AV0Z60220518
Keywords : Neoparamoeba * Diadema aff. antillarum * SSU rDNA phylogeny * Tenerife
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 2.201, year: 2011

A total of 109 sea urchins from 3 species collected in 2 localities off the coast of Tenerife Island, Spain, were examined for the presence of free-living amoebae in their coelomic fluid. Amoeba trophozoites were isolated exclusively from moribund individuals of long-spined sea urchins Diadema aff. antillarum (Philippi) (Echinoidea, Echinodermata) that manifested lesions related to sea urchin bald disease on their tests (16 out of 56 examined). No amoebae were detected in Arbacia lixula (L.) and Paracentrotus lividus (Lamarck). From the former sea urchin species, 8 strains, established from 10 primary isolates, were identified as Neoparamoeba branchiphila Dykova et al., 2005 using morphological and molecular methods. Results of this study justify the hypothesis that free-living amoebae play an opportunistic role in D. aff. antillarum mortality. The enlargement of the dataset of SSU rDNA sequences brought new insight into the phylogeny of Neoparamoeba species.
Permanent Link: http://hdl.handle.net/11104/0200144