The simple and sensitive measurement of malondialdehyde in selected specimens of biological origin and some feed by reversed phase high performance liquid chromatography

0364545 - ÚŽFG 2012 RIV NL eng J - Journal Article
Czauderna, M. - Kowalczyk, J. - Marounek, Milan
The simple and sensitive measurement of malondialdehyde in selected specimens of biological origin and some feed by reversed phase high performance liquid chromatography.
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 879, č. 23 (2011), s. 2251-2258. ISSN 1570-0232. E-ISSN 1873-376X
Institutional research plan: CEZ:AV0Z50450515
Keywords : Malondialdehyde * 2,4-Dinitrophenylhydrazine * High performance liquid chromatography
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.888, year: 2011

A method for the determination of malondialdehyde (MDA) concentrations in specimens of animal tissues and feed has been developed using high performance liquid chromatography. The MDA concentration in acidified urine samples was determined after its conversion with 2,4-dinitrophenylhydrazine (DNPH) to a hydrazone (MDA-DNPH). Samples of blood plasma, muscle, liver and feed were prepared by saponification followed by derivatisation with DNPH to MDA-DNPH. The MDA concentration in chicken and hen feed samples was analysed after saponification and derivatisation followed by extractions with hexane. The free MDA in plasma samples was determined after deproteinization followed by derivatisation of MDA with DNPH. The chromatographic separation of MDA-DNPH samples was conducted using Phenomenex C(18)-columns (Synergi 2.5 mu m, Hydro-RP, 100 angstrom, the length of 100 mm) with an inner diameter of 2 or 3 mm.
Permanent Link: http://hdl.handle.net/11104/0200008