Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog Pelophylax esculentus by Genomic in situ Hybridization

0364474 - ÚŽFG 2012 RIV CH eng J - Journal Article
Zalésna, A. - Choleva, Lukáš - Ogielska, M. - Rábová, Marie - Marec, František - Ráb, Petr
Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog Pelophylax esculentus by Genomic in situ Hybridization.
Cytogenetic and Genome Research. Roč. 134, č. 3 (2011), s. 206-212. ISSN 1424-8581. E-ISSN 1424-859X
R&D Projects: GA MŠMT LC06073; GA ČR GA523/09/2106
Institutional research plan: CEZ:AV0Z50450515; CEZ:AV0Z50070508
Keywords : Amphibia * Chromosomes * Genomic in situ hybridization (GISH)
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.533, year: 2011

The Western Palearctic water frogs Pelophylax ridibundus and P. lessonae were identified as parental (sexual) species and P. esculentus as their interspecific, hybridogenetically reproducing hybrid with hemiclonal heredity. We used genomic in situ hybridization (GISH) to identify parental chromosomes of P. lessonae and P. ridibundus in diploid P. esculentus karyotypes (2n = 26). GISH probes were made by fluorochrome labeling of total genomic DNA extracted from the sexual progenitors. The labeled probe from one species was hybridized to chromosomes of P. esculentus in the presence of excess of unlabeled genomic DNA from the other species. Thus, the P. lessonae probe was blocked by P. ridibundus unlabeled DNA, and vice versa. We successfully discriminated each of the 13 respective parental chromosomes in metaphase complements of the hybrids according to species-specific hybridization signals.
Permanent Link: http://hdl.handle.net/11104/0199953