Long-term cold acclimation extends survival time at 0°C and modifies the metabolomic profiles of the larvae of the fruit fly Drosophila melanogaster

0364369 - BC 2012 RIV US eng J - Journal Article
Košťál, Vladimír - Korbelová, J. - Rozsypal, J. - Zahradníčková, Helena - Cimlová, Jana - Tomčala, Aleš - Šimek, Petr
Long-term cold acclimation extends survival time at 0°C and modifies the metabolomic profiles of the larvae of the fruit fly Drosophila melanogaster.
PLoS ONE. Roč. 6, č. 9 (2011), e25025. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GA206/07/0269; GA ČR GA203/09/2014
Institutional research plan: CEZ:AV0Z50070508
Keywords : Drosophila melanogaster
Subject RIV: ED - Physiology
Impact factor: 4.092, year: 2011

We found that long-term cold acclimation considerably improved cold tolerance in fully grown third-instar larvae of D. melanogaster. A comparison of the larvae acclimated at constant 25°C with those acclimated at constant 15°C followed by constant 6°C for 2 d (15°C→6°C) showed that long-term cold acclimation extended the lethal time for 50% of the population (Lt50) during exposure to constant 0°C as much as 630-fold (from 0.137 h to 86.658 h). Such marked physiological plasticity in Lt50 (in contrast to LLT) suggested that chronic indirect chilling injury at 0°C differs from that caused by cold shock. Long-term cold acclimation modified the metabolomic profiles of the larvae. Accumulations of proline (up to 17.7 mM) and trehalose (up to 36.5 mM) were the two most prominent responses. In addition, restructuring of the glycerophospholipid composition of biological membranes was observed.
Permanent Link: http://hdl.handle.net/11104/0199868