Immunomagnetic sulfonated hypercrosslinked polystyrene microspheres for electrochemical detection of proteins

0364363 - ÚMCH 2012 RIV GB eng J - Journal Article
Šálek, Petr - Korecká, L. - Horák, Daniel - Petrovský, Eduard - Kovářová, Jana - Metelka, R. - Čadková, M. - Bílková, Z.
Immunomagnetic sulfonated hypercrosslinked polystyrene microspheres for electrochemical detection of proteins.
Journal of Materials Chemistry. Roč. 21, č. 38 (2011), s. 14783-14792. ISSN 0959-9428
R&D Projects: GA ČR GA203/09/0857; GA ČR GA203/09/1242
Institutional research plan: CEZ:AV0Z40500505; CEZ:AV0Z30120515
Keywords : styrene * divinylbenzene * microspheres
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 5.968, year: 2011

Poly(styrene-co-divinylbenzene) microspheres of narrow size distribution were prepared by (2-hydroxypropyl)cellulose-stabilized dispersion copolymerization of styrene and divinylbenzene in a 2-methoxyethanol/ethanol mixture under continuous addition of divinylbenzene. The copolymerization was initiated with dibenzoyl peroxide. The obtained microspheres were chloromethylated and then hypercrosslinked. Their porous structure was analyzed by nitrogen adsorption and mercury porosimetry. Superparamagnetic iron oxide nanoparticles were precipitated within the pores of microspheres from Fe(II) and Fe(III) chloride solution. The Fe content in the microspheres was determined by carbon analysis, atomic absorption spectroscopy, thermogravimetric analysis. Magnetic properties of the microspheres were characterized. Sulfo groups were introduced into the microspheres to prepare an immunomagnetic electrochemical biosensor for protein detection with ovalbumin as a model substance.
Permanent Link: http://hdl.handle.net/11104/0006549