Single-cell gene-expression profiling and its potential diagnostic applications

0364328 - BTÚ 2012 RIV GB eng J - Journal Article
Stahlberg, A. - Kubista, Mikael - Aman, P.
Single-cell gene-expression profiling and its potential diagnostic applications.
Expert Review of Molecular Diagnostics. Roč. 11, č. 7 (2011), s. 735-740. ISSN 1473-7159. E-ISSN 1744-8352
R&D Projects: GA ČR(CZ) GAP303/10/1338; GA ČR(CZ) GA301/09/1752
Institutional research plan: CEZ:AV0Z50520701
Keywords : gene-expression profiling * RT-qPCR * single-cell gene-expression profiling
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.859, year: 2011

Gene-expression profiling has been successfully applied in various diagnostic applications, but its full capacity is yet to be realized. Samples are generally prepared from a mixture of different cells that are present in unknown proportions. Cells are, in many aspects, unique in their characteristics and this heterogeneity confounds the expression profile. The development of new and robust techniques to measure gene expression in single cells opens new avenues in molecular medicine. Today, gene-expression profiles of individual cells can be measured with high precision and accuracy, identifying different cell types as well as revealing heterogeneity among cells of the same kind. Here, we review practical aspects of single-cell gene-expression profiling using reverse transcription quantitative real-time PCR and its potential use in diagnostics
Permanent Link: http://hdl.handle.net/11104/0006548