Production of polyhaploids by facultatively apomictic Pilosella can result in formation of new genotypes via genome doubling

0364311 - BÚ 2012 RIV CZ eng J - Journal Article
Krahulec, František - Krahulcová, Anna - Rosenbaumová, R. - Plačková, Ivana
Production of polyhaploids by facultatively apomictic Pilosella can result in formation of new genotypes via genome doubling.
Preslia. Roč. 83, č. 3 (2011), s. 471-490. ISSN 0032-7786. E-ISSN 0032-7786
R&D Projects: GA ČR GA206/08/0890
Institutional research plan: CEZ:AV0Z60050516
Keywords : polyploid-polyhaploid-polyploid cycle * apomixis * genome doubling
Subject RIV: EF - Botanics
Impact factor: 2.521, year: 2011

Haploid parthenogenesis in facultatively apomictic Pilosella generated polyhaploid progeny (with half the maternal chromosome set) both in natural populations and garden experiments. Production of polyhaploids varied considerably among different species, hybridogenous species and hybrids. A two-step process by which new genotypes of both P. aurantiaca (tetraploid) and P. rubra (hexaploid) were formed under garden conditions during a polyploid−polyhaploid−polyploid cycle is described. In the first step, the maternal plants generated dihaploid and trihaploid F1 progeny, respectively. Consequently, the F2 progeny resulting from the second step had a double ploidy level, identical to that of the original maternal parent. The complete process was autonomous, without contribution of pollen from parent genotype. This cycle necessarilly implicates increasing homozygosity in F2 progeny when compared to original maternal polyploid plant.
Permanent Link: http://hdl.handle.net/11104/0199830