A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae

0364285 - FGÚ 2012 RIV US eng J - Journal Article
Barreto, L. - Canadell, D. - Petrezsélyová, Silvia - Navarrete, C. - Marešová, Lydie - Peréz-Valle, J. - Herrera, R. - Olier, I. - Giraldo, J. - Sychrová, Hana - Yenush, L. - Ramos, J. - Ariňo, J.
A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae.
Eukaryotic Cell. Roč. 10, č. 9 (2011), s. 1241-1250. ISSN 1535-9778
R&D Projects: GA MŠMT(CZ) LC531; GA AV ČR(CZ) IAA500110801
Institutional research plan: CEZ:AV0Z50110509
Keywords : Potassium homeostasis * Saccharomyces cerevisiae * genomewide screen
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.604, year: 2011

Here, we describe a genome-wide screen for mutants that present altered tolerance to hygromycin B, spermine and tetramethylammonium. Two hundred twenty six mutant strains displayed altered tolerance to all three drugs (202 hypersensitive and 24 hypertolerant) and more than 50% presented a strong or moderate growth defect at limiting potassium (1 mM). Functional groups such as protein kinases and phosphatases, intracellular trafficking, transcription or cell cycle and DNA processing were enriched. Essentially, our screen has identified a substantial number of genes which were not previously described to play a direct or indirect role in potassium homeostasis. A subset of 27 representative mutants were selected and subjected to diverse biochemical tests that, in some cases, allow us to postulate the basis for the observed phenotypes
Permanent Link: http://hdl.handle.net/11104/0199807