Cell Plate Restricted Association of DRP1A and PIN Proteins Is Required for Cell Polarity Establishment in Arabidopsis

0364093 - ÚEB 2012 RIV GB eng J - Journal Article
Mravec, J. - Petrášek, Jan - Li, N. - Boeren, S. - Karlova, R. - Kitakura, S. - Pařezová, Markéta - Naramoto, S. - Nodzyński, T. - Dhonukshe, P. - Bednarek, S. Y. - Zažímalová, Eva - de Vries, S. - Friml, J.
Cell Plate Restricted Association of DRP1A and PIN Proteins Is Required for Cell Polarity Establishment in Arabidopsis.
Current Biology. Roč. 21, č. 12 (2011), s. 1055-1060. ISSN 0960-9822. E-ISSN 1879-0445
R&D Projects: GA MŠMT(CZ) LC06034
Grant - others:European Fund for Regional Development(CZ) CZ.2.16/3.1.00/21159
Institutional research plan: CEZ:AV0Z50380511
Keywords : PLANT DEVELOPMENT * PLASMA-MEMBRANE * DYNAMIN
Subject RIV: ED - Physiology
Impact factor: 9.647, year: 2011

The polarized transport of the phytohormone auxin, which is crucial for the regulation of different stages of plant development, depends on the asymmetric plasma membrane distribution of the PIN-FORMED (PIN) auxin efflux carriers. The PIN polar localization results from clathrin-mediated endocytosis (CME) from the plasma membrane and subsequent polar recycling. The Arabidopsis genome encodes two groups of dynamin-related proteins (DRPs) that show homology to mammalian dynamin-a protein required for fission of endocytic vesicles during CME. Here we show by coimmunoprecipitation (coIP), bimolecular fluorescence complementation (BiFC), and Forster resonance energy transfer (FRET) that members of the DRP1 group closely associate with PIN proteins at the cell plate. Localization and phenotypic analysis of novel drp1 mutants revealed a requirement for DRP1 function in correct PIN distribution and in auxin-mediated development.
Permanent Link: http://hdl.handle.net/11104/0199664