The use of oligoperoxide-coated magnetic nanoparticles to label stem cells

0363241 - ÚMCH 2012 RIV US eng J - Journal Article
Šponarová, Daniela - Horák, Daniel - Trchová, Miroslava - Jendelová, Pavla - Herynek, V. - Mitina, N. - Zaichenko, A. - Stoika, R. - Lesný, Petr - Syková, Eva
The use of oligoperoxide-coated magnetic nanoparticles to label stem cells.
Journal of Biomedical Nanotechnology. Roč. 7, č. 3 (2011), s. 384-394. ISSN 1550-7033. E-ISSN 1550-7041
R&D Projects: GA ČR GA203/09/1242; GA ČR GAP503/10/0664; GA MŠMT 1M0538; GA AV ČR KAN201110651; GA AV ČR(CZ) KAN401220801
Institutional research plan: CEZ:AV0Z40500505; CEZ:AV0Z50390703
Keywords : magnetic * nanoparticles * stem cells
Subject RIV: FH - Neurology
Impact factor: 4.216, year: 2011

Iron oxide nanoparticles obtained by the coprecipitation of Fe(II) and Fe(III) salts and oxidation were coated with a novel poly(vinyl acetate-co-5-tert-(butylperoxy)-5-methylhex-1-en-3-yne-co-butyl acrylate-co-maleic anhydride) (PVBM) oligomer to ensure colloidal stability. The magnetic nanoparticles were thoroughly characterized by a range of physico-chemical methods, which proved the presence of the coating on the particles. Experiments with rat mesenchymal stem cells (rMSCs) confirmed that PVBM-coated γ-Fe2O3 nanoparticles were not cytotoxic and that the average efficiency of stem cell labeling was good and comparable to that obtained with commercial agents. The cells labeled with PVBM-coated γ-Fe2O3 nanoparticles displayed excellent contrast on magnetic resonance (MR) images. Such particles are thus promising for in vivo MR imaging of transplanted cells. Moreover, PVBM offers possibility of additional modification by grafting compounds that reduce non-specific protein adsorption.
Permanent Link: http://hdl.handle.net/11104/0199248