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Fluorescence method for determining the mechanism and speed of action of surface-active drugs on yeast cells
- 1.0362824 - MBÚ 2012 RIV US eng J - Journal Article
Kodedová, M. - Sigler, Karel - Lemire, B. D. - Gášková, Dana
Fluorescence method for determining the mechanism and speed of action of surface-active drugs on yeast cells.
Biotechniques. Roč. 50, č. 1 (2011), 58-63. ISSN 0736-6205. E-ISSN 1940-9818
R&D Projects: GA MŠMT 1M0570
Institutional research plan: CEZ:AV0Z50200510
Keywords : fluorescent dye diS-C(3)(3) * membrane potential * surface-active drug
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.669, year: 2011
New antifungal agents are needed to treat life-threatening fungal infections, particularly with the development of resistance. Surface-active antifungals have the advantages of minimizing host toxicity and the emergence of drug resistance. We have developed a time-dependent drug exposure assay that allows us to rapidly investigate the mechanism of surface-active antifungal drug action. The assay uses a multidrug pump-deficient strain of Saccharomyces cerevisiae and the potentiometric dye 3,3-dipropylthiacarbocyanine iodide [diS-C3(3)] and can assess whether cells are depolarized, hyperpolarized, or permeabilized by drug exposure
Permanent Link: http://hdl.handle.net/11104/0199022
Number of the records: 1