Identification of thylakoid membrane thermal transitions in Synechocystis sp. PCC6803 photosynthetic mutants

0362733 - MBÚ 2012 RIV NL eng J - Journal Article
Laczkó-Dobos, H. - Todinova, S. J. - Sozer, O. - Komenda, Josef - Kis, M. - Sallai, A. - Dobrikova, A. G. - Ughy, B. - Debreczeny, M. - Gombos, Z. - Apostolova, E. L. - Domonkos, I.
Identification of thylakoid membrane thermal transitions in Synechocystis sp. PCC6803 photosynthetic mutants.
Photosynthesis Research. Roč. 107, č. 3 (2011), 237-246. ISSN 0166-8595. E-ISSN 1573-5079
Institutional research plan: CEZ:AV0Z50200510
Keywords : Differential scanning calorimetry * Cyanobacterium * Photosynthesis
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.243, year: 2011

Differential scanning calorimetry (DSC) was used for identification of photosynthetic complexes on the basis of their calorimetric transitions. Annotation of thermal transitions was carried out with thylakoid membranes isolated from various photosynthetic mutants of Synechocystis sp. PCC6803. The thylakoid membranes exhibited seven major DSC bands between 40 and 85 C. The heat sorption curves were analyzed both by mathematical deconvolution of the overall endotherms and by a subsequent annealing procedure. The successive annealing procedure proved to be more reliable technique than mathematical deconvolution in assigning thermal transitions
Permanent Link: http://hdl.handle.net/11104/0198972