Method of cultivation of human mesenchymal stem cells, particularly for the treatment of non-healing fractures, and bioreactor for carrying out this cultivation method

0361674 - ÚMCH 2012 RIV eng P - Patent Document
Pytlík, R. - Hofman, P. - Trč, T. - Stehlík, D. - Soukup, T. - Kobylka, P. - Klener, P. - Rypáček, František - Mulinková, Katarína
Method of cultivation of human mesenchymal stem cells, particularly for the treatment of non-healing fractures, and bioreactor for carrying out this cultivation method.
2011. Owner: Univerzita Karlova v Praze - Ústav hematologie a krevní transfuze - Ústav makromolekulární chemie AV ČR, v. v. i. Date of the patent acceptance: 20.07.2011. Patent Number: EP1999250
R&D Projects: GA MZd ND7448
Institutional research plan: CEZ:AV0Z40500505
Keywords : mesenchymal stem cells * cultivation * bone-fracture treatment
Subject RIV: FJ - Surgery incl. Transplants
http://worldwide.espacenet.com/publicationDetails/originalDocument?FT=D&date=20110720&DB=EPODOC&locale=en_EP&CC=EP&NR=1999250B1&KC=B1

The invention relates to novel method of cultivation of mesenchymal stem cells, wherein after aseptic separation of mononuclear cells from the marrow blood, the said cells are seeded in low density into sterile plastic cultivation vessels and cultivated for about one to three weeks in CellGroTM Hematopoietic Stem cell Medium, certified for clinical use, with addition of 10% human serum and supplements, wherein the supplements are added at least once in the course of cultivation, without removal of hematopoietic cells and without medium exchange during the cultivation procedure, without any interference with the closed cultivation system, under standard conditions for the cultivation of tissue culture.
Permanent Link: http://hdl.handle.net/11104/0198940