Polymerase synthesis of modified DNA containing cytosine in major groove

0361573 - ÚOCHB 2012 CZ eng C - Conference Paper (international conference)
Kielkowski, Pavel - Hocek, Michal
Polymerase synthesis of modified DNA containing cytosine in major groove.
Chemistry of Nucleic Acid Components. 15th Symposium. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i., 2011 - (Hocek, M.), s. 359-360. Collection Symposium Series, 12. ISBN 978-80-86241-37-1.
[Chemistry of Nucleic Acid Components /15./. Český Krumlov (CZ), 05.06.2011-10.06.2011]
R&D Projects: GA MŠMT LC512; GA ČR GA203/09/0317
Institutional research plan: CEZ:AV0Z40550506
Keywords : DNA * polymerase * cytosine
Subject RIV: CC - Organic Chemistry

(Cytosine-5-yl)ethynyl derivatives of pyrimidine and 7-deazaadenine 2-deoxyribonucleosides and nucleoside triphosphates (dNTPs) were prepared by aqueous Sonogashira coupling. All modified dNTPs were excellent substrates for DNA polymerases to give DNA bearing acetylene- linked cytosine(s) in the major groove.
Permanent Link: http://hdl.handle.net/11104/0198861