Effect of pH on the denitrifying enzyme activity in pasture soils in relation to the intrinsic differences in denitrifier communities

0361563 - BC 2012 RIV CZ eng J - Journal Article
Čuhel, Jiří - Šimek, Miloslav
Effect of pH on the denitrifying enzyme activity in pasture soils in relation to the intrinsic differences in denitrifier communities.
Folia Microbiologica. Roč. 56, č. 3 (2011), s. 230-235. ISSN 0015-5632. E-ISSN 1874-9356
R&D Projects: GA MŠMT LC06066; GA AV ČR IAA600660605
Institutional research plan: CEZ:AV0Z60660521
Keywords : pH * denitrifying enzyme activity * pasture soils
Subject RIV: EH - Ecology, Behaviour
Impact factor: 0.677, year: 2011

The effects of pH on denitrifying enzyme activity (DEA) and on the ratio of the denitrification products, N₂O and N₂, were determined in three pasture soils differing in cattle impact. The linkage between intrinsic differences in the denitrifying communities and pH effects on relative N₂O production was also assessed. Soil pH values were adjusted just before DEA determination to obtain soil slurries with a range of pH values. The intrinsic differences in the denitrifier communities were assessed by measuring the kinetic constants of NO₃^- and N₂O reductions. DEA for all three soils was highest at pH 8.4, regardless of native soil pH. Because DEA has typically been measured at native soil pH, our results suggest that DEA might have been underestimated in many previous studies.
Permanent Link: http://hdl.handle.net/11104/0198854