Radiation damage to DNA-protein complexes

0361498 - ÚJF 2012 RIV GB eng J - Journal Article
Spotheim-Maurizot, M. - Davídková, Marie
Radiation damage to DNA-protein complexes.
Journal of Physics: Conference Series. Roč. 261, zima (2011), s. 1-10. ISSN 1742-6588. E-ISSN 1742-6596.
[COST Chemistry CM0603-MELUSYN Joint Meeting Damages Induced in Biomolecules by Low and High Energy Radiations. Paříž, 09.03.2010-12.03.2010]
R&D Projects: GA AV ČR IAA1048103; GA AV ČR KJB4048401; GA MŠMT 1P05OC085; GA MŠMT OC09012; GA AV ČR IAB1048901
Institutional research plan: CEZ:AV0Z10480505
Keywords : radiolysis * molecular-dynamics simulation * hydroxyl radical attack * induced strand breakage
Subject RIV: BO - Biophysics

We review here the advances in understanding the effects of ionizing radiations on DNA, proteins and their complexes. A model of simulation of the reaction of these radicals with the macromolecules (called RADACK) was developed and was used for calculating the probabilities of damage of each constituent of DNA or proteins (nucleotide or amino-acid). The calculations allowed to draw conclusions from electrophoresis, mutagenesis, spectroscopic (fluorescence, circular dichroism) and mass spectrometry experiments. Thus we have shown that the extent and location of the lesions are strongly dependent on the 3D structure of the macromolecules, which in turns is modulated by their sequence and by the binding of some ligands.
Permanent Link: http://hdl.handle.net/11104/0198799