Radiation damage to DNA in DNA-protein complexes

0361467 - ÚJF 2012 RIV NL eng J - Journal Article
Spotheim Maurizot, M. - Davídková, Marie
Radiation damage to DNA in DNA-protein complexes.
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. Roč. 711, 1-2 (2011), s. 41-48. ISSN 0027-5107
Institutional research plan: CEZ:AV0Z10480505
Keywords : DNA-protein complex * ionizing radiation * molecular structure
Subject RIV: BO - Biophysics
Impact factor: 2.850, year: 2011

The most aggressive product of water radiolysis, the hydroxyl (OH) radical, is responsible for the indirect effect of ionizing radiations on DNA in solution and aerobic conditions. Monte-Carlo based model RADACK, allows calculating the relative probability of damage of each nucleotide of DNA irradiated alone or in complexes with proteins. The confrontation of the calculated values with the results of the radiolytic footprinting experiments together with molecular modeling calculations show that: (1) the extent and location of the lesions are strongly dependent on the structure of DNA, which in turns is modulated by the base sequence and by the binding of proteins and (2) the regions in contact with the protein can be protected against the attack by the hydroxyl radicals via masking of the binding site and by scavenging of the radicals.
Permanent Link: http://hdl.handle.net/11104/0198775