Breakdown of the Blood-Brain Barrier during Tick-Borne Encephalitis in Mice Is Not Dependent on CD8(+) T-Cells

0361425 - BC 2012 RIV US eng J - Journal Article
Růžek, Daniel - Salát, Jiří - Singh, S. K. - Kopecký, Jan
Breakdown of the Blood-Brain Barrier during Tick-Borne Encephalitis in Mice Is Not Dependent on CD8(+) T-Cells.
PLoS ONE. Roč. 6, č. 5 (2011), e20472. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GPP302/10/P438; GA ČR GAP502/11/2116; GA MŠMT(CZ) LC06009
Institutional research plan: CEZ:AV0Z60220518
Keywords : CENTRAL-NERVOUS-SYSTEM * WEST-NILE-VIRUS * MICROVASCULAR ENDOTHELIAL-CELLS * TIGHT JUNCTION PROTEINS * MULTIPLE-SCLEROSIS * INFLAMMATORY RESPONSE * PERMEABILITY CHANGES
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.092, year: 2011

Tick-borne encephalitis (TBE) virus causes severe encephalitis with serious sequelae in humans. The disease is characterized by fever and debilitating encephalitis that can progress to chronic illness or fatal infection. In this study, changes in permeability of the blood-brain barrier (BBB) in two susceptible animal models (BALB/c, and C57Bl/6 mice) infected with TBE virus were investigated at various days after infection by measuring fluorescence in brain homogenates after intraperitoneal injection of sodium fluorescein, a compound that is normally excluded from the central nervous system. We demonstrate here that TBE virus infection, in addition to causing fatal encephalitis in mice, induces considerable breakdown of the BBB. Breakdown of the BBB was also observed in mice deficient in CD8(+) T-cells, indicating that these cells are not necessary for the increase in BBB permeability that occurs during TBE.
Permanent Link: http://hdl.handle.net/11104/0198737