Quantification of methyl thiocyanate in the headspace of Pseudomonas aeruginosa cultures and in the breath of cystic fibrosis patients by selected ion flow tube mass spectrometry

0361411 - ÚFCH JH 2012 RIV GB eng J - Journal Article
Shestivska, Violetta - Němec, A. - Dřevínek, P. - Sovová, Kristýna - Dryahina, Kseniya - Španěl, Patrik
Quantification of methyl thiocyanate in the headspace of Pseudomonas aeruginosa cultures and in the breath of cystic fibrosis patients by selected ion flow tube mass spectrometry.
Rapid Communications in Mass Spectrometry. Roč. 25, č. 18 (2011), s. 2459-2467. ISSN 0951-4198. E-ISSN 1097-0231
R&D Projects: GA ČR GA203/09/0256; GA ČR GP203/09/P172
Institutional research plan: CEZ:AV0Z40400503
Keywords : pseudomonas aeruginosa cultures * flow tube mass spectrometry * methyl thiocyanate
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 2.790, year: 2011

Infection by Pseudomonas aeruginosa (PA) is a major cause of morbidity and mortality in patients with cystic fibrosis (CF). Breath analysis could potentially be a useful diagnostic of such infection, and analyses of volatile organic compounds (VOCs) emitted from PA cultures are an important part of the search for volatile breath markers of PA lung infection. Our pilot experiments using solid-phase microextraction, SPME and gas chromatography/mass spectrometric (GC/MS) analyses of volatile compounds produced by PA strains indicated a clear presence of methyl thiocyanate. This provided a motivation to develop a method for real-time online quantification of this compound by selected ion flow tube mass spectrometry, SIFT-MS.
Permanent Link: http://hdl.handle.net/11104/0006512