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Seasonal changes of free amino acids and thermal hysteresis in overwintering heteropteran insect, Pyrrhocoris apterus

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    0361246 - BC 2012 RIV US eng J - Journal Article
    Košťál, Vladimír - Renault, D. - Rozsypal, Jan
    Seasonal changes of free amino acids and thermal hysteresis in overwintering heteropteran insect, Pyrrhocoris apterus.
    Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology. Roč. 160, č. 2 (2011), s. 245-251. ISSN 1095-6433. E-ISSN 1531-4332
    R&D Projects: GA ČR GA206/07/0269
    Institutional research plan: CEZ:AV0Z50070508
    Keywords : diapause * cold tolerance * ice nucleation
    Subject RIV: ED - Physiology
    Impact factor: 2.235, year: 2011

    We sampled the adults monthly in the field during the cold season 2008-2009 and found active thermal hysteresis factors (THFs) in hemolymph of winter-sampled adults. The hysteresis between the equilibrium melting and freezing points ranged from 0.18°C to 0.30°C. The total free amino acid pool almost doubled during winter time. The sum concentrations of 27 free amino acids ranged between 35 – 40 mM in whole body water and 40 – 45 mM in hemolymph during December - February. Two amino acids, Pro and α-Ala most significantly contributed to the seasonal increase, while Gln showed the most dramatic seasonal decrease.
    Permanent Link: http://hdl.handle.net/11104/0198606

     
     
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