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Synthesis of acetylene linked double-nucleobase nucleos(t)ide building blocks and polymerase construction of DNA containing cytosines in the major groove
- 1.0360994 - ÚOCHB 2012 RIV US eng J - Journal Article
Kielkowski, Pavel - Pohl, Radek - Hocek, Michal
Synthesis of acetylene linked double-nucleobase nucleos(t)ide building blocks and polymerase construction of DNA containing cytosines in the major groove.
Journal of Organic Chemistry. Roč. 76, č. 9 (2011), s. 3457-3462. ISSN 0022-3263. E-ISSN 1520-6904
R&D Projects: GA MŠMT LC512; GA ČR GA203/09/0317
Institutional research plan: CEZ:AV0Z40550506
Keywords : nucleoside triphosphates * functionalized DNA * cross-coupling reactions * nucleic-acids
Subject RIV: CC - Organic Chemistry
Impact factor: 4.450, year: 2011
Cytosin-5-yl ethynyl derivatives of pyrimidine and 7-deazaadenine 2-deoxyribonucleosides and nucleoside triphosphates (dNTPs) were prepared in one step by the aqueous Sonogashira coupling of unprotected halogenated nucleos(t)ides with 5-ethynylcytosine. The modified dNTPs were good substrates for DNA polymerases suitable for primer extension or PCR construction of DNA bearing acetylene-linked cytosine(s) in the major groove mimicking the flipped-out nucleotide.
Permanent Link: http://hdl.handle.net/11104/0198417
Number of the records: 1