High-yield continuous production of nicotinic acid via nitrile hydratase–amidase cascade reactions using cascade CSMRs

0360788 - MBÚ 2012 RIV US eng J - Journal Article
Cantarella, L. - Gallifuoco, A. - Malandra, A. - Martínková, Ludmila - Spera, A. - Cantarella, M.
High-yield continuous production of nicotinic acid via nitrile hydratase–amidase cascade reactions using cascade CSMRs.
Enzyme and Microbial Technology. Roč. 48, 4-5 (2011), 345-350. ISSN 0141-0229. E-ISSN 1879-0909
R&D Projects: GA MŠMT OC09046
Institutional research plan: CEZ:AV0Z50200510
Keywords : Nitrile hydratase-amidase cascade system * 3-Cyanopyridine bioconversion * Nicotinic acid
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.367, year: 2011

High yields of nicotinic acid from 3-cyanopyridine bioconversion were obtained by exploiting the in situ nitrile hydratase–amidase enzymatic cascade system of Microbacterium imperiale CBS 498-74. Experiments were carried out in continuously stirred tank UF-membrane bioreactors (CSMRs) arranged in series. This reactor configuration enables both enzymes, involved in the cascade reaction, to work with optimized kinetics, without any purification, exploiting their differing temperature dependences. To this end, the first CSMR, optimized for the properties of the NHase, was operated (i) at low temperature (5◦C), limiting inactivation of the more fragile enzyme, nitrile hydratase, (ii) with a high residence time (24h) to overcome reaction rate limitation
Permanent Link: http://hdl.handle.net/11104/0198255