Development of microsatellite markers for a diving duck, the common pochard (Aythya ferina)

0360723 - ÚBO 2012 RIV NL eng J - Journal Article
Šťovíček, O. - Čížková, Dagmar - Yang, L. - Albrecht, Tomáš - Heckel, G. - Vyskočilová, M. - Kreisinger, J.
Development of microsatellite markers for a diving duck, the common pochard (Aythya ferina).
Conservation genetics resources. Roč. 3, č. 3 (2011), s. 573-576. ISSN 1877-7252. E-ISSN 1877-7260
R&D Projects: GA MŠMT LC06073; GA AV ČR KJB601110803; GA MŠMT LC522
Institutional research plan: CEZ:AV0Z60930519
Keywords : Waterfowl * Conspecific brood parasitism * Extra pair paternity * Conservation genetic
Subject RIV: EG - Zoology
Impact factor: 0.485, year: 2011

Ten polymorphic microsatellites for the common pochard were isolated from microsatellite enriched libraries. Seven microsatellites were obtained based on cross-species amplification. These 17 microsatellites exhibited polymorphism within a population sample, with numbers of alleles per locus ranging from 2 to 17 and expected heterozygosities from 0.053 to 0.916. These markers will be helpful for studying reproductive strategies in the pochard, as well as in addressing population genetic questions. Four of the microsatellite loci showed significant departures from Hardy-Weinberg equilibrium and/or high estimates of null allele frequencies in our study population. Hence, they should be used with caution.
Permanent Link: http://hdl.handle.net/11104/0198207