Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10

0360698 - MBÚ 2012 RIV GB eng J - Journal Article
Kaplan, Ondřej - Bezouška, Karel - Plíhal, Ondřej - Ettrich, Rüdiger - Kulik, Natallia - Vaněk, Ondřej - Kavan, Daniel - Benada, Oldřich - Malandra, Anna - Šveda, Ondřej - Veselá, Alicja Barbara - Rinágelová, Anna - Slámová, Kristýna - Cantarella, M. - Felsberg, Jürgen - Dušková, Jarmila - Dohnálek, Jan - Kotík, Michael - Křen, Vladimír - Martínková, Ludmila
Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10.
BMC Biotechnology. Roč. 11, č. 2 (2011), s. 1-15. E-ISSN 1472-6750
R&D Projects: GA AV ČR IAA500200708; GA MŠMT(CZ) LC06010; GA MŠMT OC09046; GA ČR GD305/09/H008; GA ČR GA310/09/1407
Institutional research plan: CEZ:AV0Z50200510; CEZ:AV0Z40500505; CEZ:AV0Z60870520
Keywords : MULTIPLE SEQUENCE ALIGNMENT * RHODOCOCCUS-RHODOCHROUS J1 * CRYSTAL-STRUCTURE
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.349, year: 2011

Nitrilases attract increasing attention due to their utility in the mild hydrolysis of nitriles. According to activity and gene screening, filamentous fungi are a rich source of nitrilases distinct in evolution from their widely examined bacterial counterparts. However, fungal nitrilases have been less explored than the bacterial ones. Nitrilases are typically heterogeneous in their quaternary structures, forming short spirals and extended filaments, these features making their structural studies difficult. The nitrilase from Aspergillus niger K10 is highly homologous with proteins deduced from gene sequencing in Aspergillus and Penicillium genera
Permanent Link: http://hdl.handle.net/11104/0198187