DNA adducts and oxidative DNA damage induced by organic extracts from PM2.5 in an acellular assay

0360452 - ÚEM 2012 RIV IE eng J - Journal Article
Topinka, Jan - Rössner ml., Pavel - Milcová, Alena - Schmuczerová, Jana - Švecová, Vlasta - Šrám, Radim
DNA adducts and oxidative DNA damage induced by organic extracts from PM2.5 in an acellular assay.
Toxicology Letters. Roč. 202, č. 3 (2011), s. 186-192. ISSN 0378-4274. E-ISSN 1879-3169
R&D Projects: GA MŠMT 2B08005; GA MŽP(CZ) SP/1B3/8/08
Institutional research plan: CEZ:AV0Z50390512
Keywords : air pollution * genotoxicity * DNA adducts
Subject RIV: DN - Health Impact of the Environment Quality
Impact factor: 3.230, year: 2011
http://arl-repository.lib.cas.cz/uloziste_av/UEM-P/cav_un_epca-0360452_01.pdf

The genotoxic acitivity of complex mixtures of organic extracts from urban air particles (PM2.5) collected in various localities of the Czech Republic with different levels of air pollution was compared. As markers of genotoxic potential, DNA adduct levels and oxidative DNA damage (8-oxodG levels) induced by EOMs in an acellular assay of calf thymus DNA coupled with 32P-postlabeling (DNA adducts) and ELISA (8-oxodG) were employed. The results indicate that the industrial emissions prevailing in Ostrava-Bartovice represent a substantially higher genotoxic risk that mostly traffic-related emissions in Ostrava-Poruba. B[a]P and c-PAH contents in EOMs are the most important factors relating to their genotoxic potential.
Permanent Link: http://hdl.handle.net/11104/0198004