A comprehensive study in triblock copolymer membrane interaction

0360337 - ÚFCH JH 2012 RIV NL eng J - Journal Article
Pembouong, G. - Morellet, N. - Kral, Teresa - Hof, Martin - Scherman, D. - Bureau, M.-F. - Mignet, N.
A comprehensive study in triblock copolymer membrane interaction.
Journal of Controlled Release. Roč. 151, č. 1 (2011), s. 57-64. ISSN 0168-3659. E-ISSN 1873-4995
R&D Projects: GA AV ČR IAA400400621; GA MŠMT(CZ) LC06063
Institutional research plan: CEZ:AV0Z40400503
Keywords : poloxamer * pluronic * liposomes
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 5.732, year: 2011

Poloxamers are triblock copolymers made of poly(ethylene glycol)-(poly(propylene glycol))-poly(ethylene glycol). They have been shown to enhance gene transfer in the muscle, and co-administration of polymers and DNA appeared to be crucial to obtain this effect. It is questionable then if some interaction occurs between polymers and DNA. Polymer interaction with membranes represents a second crucial point due to the central hydrophobic part of the triblock copolymers. Besides, the question of the polymer spanning or adsorbing to the surface has not been solved by now. We addressed these issues by means of sensitive techniques that allowed working in diluted conditions and gaining in comprehension of gene transfection. By means of simultaneous time-correlated single-photon counting and fluorescence correlation spectroscopy, we have shown that the diffusion time of a single DNA molecule and PicoGreen lifetime was not altered in the presence of the triblock copolymer L64.
Permanent Link: http://hdl.handle.net/11104/0197913