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Three metallothionein isoforms and sequestration of intracellular silver in the hyperaccumulator Amanita strobiliformis
- 1.0360138 - ÚJF 2012 RIV US eng J - Journal Article
Osobova, M. - Urban, V. - Jedelský, P.L. - Borovička, Jan - Gryndler, Milan - Ruml, T. - Kotrba, P.
Three metallothionein isoforms and sequestration of intracellular silver in the hyperaccumulator Amanita strobiliformis.
New Phytologist. Roč. 190, č. 4 (2011), s. 916-926. ISSN 0028-646X. E-ISSN 1469-8137
R&D Projects: GA AV ČR IAA600480801
Institutional research plan: CEZ:AV0Z10480505; CEZ:AV0Z50200510
Keywords : ectomycorrhizal fungi * fungal metallothionein * heavy metal
Subject RIV: DD - Geochemistry
Impact factor: 6.645, year: 2011
P>Metallothioneins (MTs) are cysteine-rich peptides involved in heavy metal tolerance of many eukaryotes. Here, we examined their involvement in intracellular binding of silver (Ag) in the ectomycorrhizal fungus Amanita strobiliformis. The Ag complexes and their peptide ligands were characterized using chromatography and mass spectrometry. The full-length coding sequences obtained from a cDNA library were used for complementation assays in yeast mutant strains.
Permanent Link: http://hdl.handle.net/11104/0197763
Number of the records: 1