The effect of passage through the gut of earthworms (Annelida: Lumbricidae) on the diversity of archaea and bacteria

0360066 - BC 2012 RIV CZ eng J - Journal Article
Koubová, Anna - Knapp, B.A. - Insam, H. - Pižl, Václav - Elhottová, Dana
The effect of passage through the gut of earthworms (Annelida: Lumbricidae) on the diversity of archaea and bacteria.
Acta Societatis Zoologicae Bohemicae. Roč. 74, - (2010), s. 69-74. ISSN 1211-376X.
[Central European Workshop on Soil Zoology /10./. České Budějovice, 21.04.2009-24.04.2009]
R&D Projects: GA MŠMT LC06066; GA MŠMT MEB060814; GA AV ČR IAA600200704
Institutional research plan: CEZ:AV0Z60660521
Keywords : soil zoology * soil microbiology * microbial diversity
Subject RIV: EH - Ecology, Behaviour

The changes that occurred in the diversity of microbes in soil and compost during its passage through the gut of earthworms were evaluated in two epigeic earthworm species Eisenia fetida (Savigny, 1826) and E. andrei (Bouché, 1972), which inhabit forest soil and garden organic compost, respectively. A modified extraction protocol was used to extract DNA from forest soil, compost and fresh earthworm faeces. DNA was amplified in a polymerase chain reaction (PCR). The products were analyzed using denaturing gradient gel electrophoresis (DGGE) fingerprinting. Analysis of archaeal and bacterial DGGE fingerprints revealed that the diversity in both microbial domains increased after passage through the guts of E. andrei, but not E. fetida. Nevertheless, cluster and correspondence analyses revealed that the passage of soil and compost through the guts of E. fetida, E. andrei, respectively, had a significant effect on the composition of the archaea.
Permanent Link: http://hdl.handle.net/11104/0197705