Land use intensity controls actinobacterial community structure

0359897 - BC 2012 RIV US eng J - Journal Article
Hill, P. - Krištůfek, Václav - Dijkhuizen, L. - Boddy, Ch. - Kroetsch, D. - van Elsas, J.D.
Land use intensity controls actinobacterial community structure.
Microbial Ecology. Roč. 61, č. 2 (2011), s. 286-302. ISSN 0095-3628. E-ISSN 1432-184X
R&D Projects: GA MŠMT LC06066; GA MŠMT 2B06154
Institutional research plan: CEZ:AV0Z60660521
Keywords : actinobacterial community structure * DNA * soils
Subject RIV: EH - Ecology, Behaviour
Impact factor: 2.912, year: 2011

Actinobacteria are major producers of secondary metabolites; however, it is unclear how they are distributed in the environment. DNA was extracted from forest, pasture and cultivated soils, street sediments (dust and material in place), and sediments affected by animal activity (e.g. guano, vermicompost) and characterised with two actinobacterial and a bacterial-specific 16S rDNA primer set. Amplicons (140/156) generated with the two actinobacterial-specific and amplicons (471) generated with bacterial-specific primers were analysed. Amplicons from actinobacterial-specific primer were disproportionately actinomycetal from animal-affected (soil) samples and street sediments and either verrucomicrobial (i.e. non-actinobacterial) and from a novel non-actinomycetal actinobacterial group for soils.
Permanent Link: http://hdl.handle.net/11104/0197589