Bioactive support for cell cultivation and potential grafting. Part1. Surface modification of 2-hydroxyethyl methacrylate hydrogels for avidin immobilization

0359861 - ÚMCH 2012 RIV US eng J - Journal Article
Hobzová, Radka - Přádný, Martin - Zhunusbekova, Nazym M. - Širc, Jakub - Guryča, Vilém - Michálek, Jiří
Bioactive support for cell cultivation and potential grafting. Part1. Surface modification of 2-hydroxyethyl methacrylate hydrogels for avidin immobilization.
E-Polymers. -, 043 (2011), s. 1-17. ISSN 1618-7229. E-ISSN 1618-7229
R&D Projects: GA AV ČR 1QS400500558
Institutional research plan: CEZ:AV0Z40500505
Keywords : hydrogel * poly(2-hydroxyethyl methacrylate) * surface modification
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 0.515, year: 2011
http://www.e-polymers.org/journal/abstract.cfm?abstract_Id=3616

Synthetic hydrogels are often used in biomedical applications as many of them are compatible with living tissue and moreover they meet the most criteria for artificial tissue properties. To enhance the cell adhesion and proliferation, it is desirable to modify the polymer surface by some bioactive compounds (e.g. saccharides, proteins). In this work, firstly carboxyl-enriched hydrogels were prepared by various methods: by copolymerization of 2 hydroxyethyl methacrylate with methacrylic acid, by hydrolysis or oxidation of poly(2 hydroxyethyl methacrylate). Subsequently, avidin was immobilized to the modified hydrogel surfaces either electrostatically through dissociated carboxylic groups or covalently through carboxylic groups activated by N-hydroxysuccinimide. Preliminary biological tests showed that hydrogels with immobilized avidin provides better adhesion and proliferation of keratinocytes compared to supports without avidin.
Permanent Link: http://hdl.handle.net/11104/0006455