Fluorescent magnetic nanoparticles for biomedical applications

0359777 - ÚMCH 2012 RIV GB eng J - Journal Article
Chekina, Nataliya - Horák, Daniel - Jendelová, Pavla - Trchová, Miroslava - Beneš, Milan J. - Hrubý, Martin - Herynek, V. - Turnovcová, Karolína - Syková, Eva
Fluorescent magnetic nanoparticles for biomedical applications.
Journal of Materials Chemistry. Roč. 21, č. 21 (2011), s. 7630-7639. ISSN 0959-9428
R&D Projects: GA MŠMT 7E09109; GA ČR GA203/09/1242; GA AV ČR KAN201110651; GA AV ČR KAN200200651
Institutional research plan: CEZ:AV0Z40500505; CEZ:AV0Z50390703
Keywords : magnetic * fluorescent * stem cells
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 5.968, year: 2011

The simultaneous combination of optical and magnetic resonance imaging (MRI) would greatly benefit in vivo disease diagnosis as well as in situ monitoring of living cells. In order to design dual detection of cells involving simultaneous imaging by fluorescent microscopy and MRI, nanoparticles with two reporters, a fluorescent dye and a superparamagnetic core, included in one particle were synthesized and characterized. The γ-Fe2O3 nanoparticles obtained by coprecipitation and oxidation were coated with silica (SiO2) or carboxymethyl chitosan (CMCS) and labeled with fluorescein isothiocyanate (FITC). The fluorescent label was covalently bound to the nanoparticles and was not quenched by the iron oxide core. The nanoparticles successfully labeled rat mesenchymal stem cells (rMSCs) in vitro. Relaxation time measurements found large amounts of iron inside the cells with FITC-labeled γ-Fe2O3–SiO2-AP nanoparticles.
Permanent Link: http://hdl.handle.net/11104/0006453